Technology Developed

 

1. Production, process, technologies developed by the Institute with credited scientist

A. ICAR- National Bureau of Agriculturally Important Microorganisms

 

Ø Establishment of National Agriculturally Important Microbial culture Collection (NAIMCC) with a total holding of 6322 cultures which includes fungi, bacteria, actinomycetes and blue green algae. All the cultures are maintained in three forms: glycerol based, mineral oil based and lyophilized.

 

Ø DNA based diagnostic kits for the identification and ecological monitoring of Bacillus, Pseudomonas, Alternaria, Colletotrichum, Fusarium udum and Macrophomina phaseolina. At present the Bureau is working for development of molecular barcodes based on signature sequences for accurate detection of the microorganisms.

 

Ø Sequenced and annotated draft genomes of Mesorhizobium ciceri strain Ca181, Pseudomonas koreensis P2, Brevibacillus borstelensis LCHU R05, Exiguobacterium profundum,Staphylococcus xylosus strain LSR_02N, Exiguobacterium profundum PHM11, Bacillus subtilis RC25, Fusarium udum F02845, Chromohalobacter selaxigenes, Pseudomonas azotoformans, Pseudomonas aeruginosa PF1, Pseudomonas aeruginosa PF3, Pseudomonas plecoglossicida PF2 and PF4

 

Ø Biopesticide formulations (4)

 

· Eco-Pesticide (Talc based bioformulation of Pseudomonas fluorescens);

· Eco-Green Fungicide (Vermi-based bioformulation of T. viride)

· Green Fungicide (Talc based bioformulation of Trichoderma harzianum)

 

All these formulations wereeffective against a number of soil and seed borne pathogens like Rhizoctonia, Sclerotium, Sclerotinia, Fusarium, Pythium, Ralstonia, Macrophomina, Bipolaris, Phoma, etc.

 

· Bio Pulse:A fly ash based bioformulation of Trichoderma harzianum and Bacillus amyloliquefaciens named ‘Bio Pulse’ has been developed and evaluated for control of Fusarium wilt in chickpea. Treatment with formulation could suppress wilt disease by 40% and increased the grain yield by 15% in chickpea on farmers’ field.

 

These formulations have been validated in the field but not commercialized as they have not been registered with CIB&RC.

 

Ø Microbial formulations for nutrient management

 

· Bio NPK: Bio NPK is a unique formulation that consists of a nitrogen fixing (Azotobacter chroococcum), P-solubilizing (Paenibacillus tylopili) and K –solubilizing (Bacillus decolorationis) bacteria. Inoculation with Bio NPK providing formulation increases the yield by 10-15% and curtails the use of costly chemical fertilizers by 25-30%. The technology has been validated for Maize at multiple (22) locations under AICRP, for fodder crops at ICAR-IGFRI, Jhansi, for Wheat at ICAR-NBAIM, Mau and under farmers field for rice and wheat. In addition the technology has given good results in citrus orchards (in Punjab), papaya (Begusarai, Bihar), ginger and turmeric (Anand, Gujarat). Technology is registered with Agrinnovate Pvt Ltd. Technology licensed to M/s Arihant Naturecrop Private Limited, Patna, Bihar

 

· BIOGROW: A consortium consisting of P solubilizing bacteria, siderophore and IAA producing bacteria for vegetable crops particularly solanaceous crops. In tomato, 25-30% increase in yield was recorded in treatment inoculated with BIOGROW. Moreover there was a significant improvement in nutritional quality as evident from enhanced content of lycopene and β-carotene in inoculated treatment as compared to control. Technology is registered with Agrinnovate Pvt Ltd.

 

· Biophos and BioPhos+ : Bio Phos and Bio Phos+ are liquid formulations of P- solubilizing bacteria containing Paenibacillus tylopiliandKluyvera cryocrescensrespectively.Phosphorus solubilising bacteria play crucial role in dissolution or insoluble source of phosphorus through secretion of organic acids and other metabolites. When applied to soil, it converts insoluble form of P to soluble form and make it available to the plant. Inoculation of P solubilizer helps to augment 15 to 20 kg P2O5 ha-1. Technology is registered with Agrinnovate Pvt Ltd.

 

· Bio Zinc:It is a liquid formulation of Zinc solubilizing bacteria. Zinc solubilising bacteria play crucial role in dissolution of insoluble source of zinc through secretion of organic acids and other metabolites. When applied to soil, it converts insoluble form of Zn to soluble form and make it available to the plant. Inoculation of Zn solubilizer helps to augment 2 to 5 kg Zn ha-1. Technology is registered with Agrinnovate Pvt Ltd.

 

· Bio Potash: It is a liquid formulation of K solubilizing bacteria and can be applied to crops like maize, potato and can provide 5-10 kg K /ha.

 

Ø Microbial consortium for composting

· Bio Compost: A consortium of four hyper lignocellulolytic fungi (Trichoderma viride, Aspergillus niger, Pleurotus florida and Phanerochaete chrysosporium) recommended for composting of lignocellulosic waste. The technology has been demonstrated in villages nearby Mau and Azamgarh.

 

Ø Liquid bioformulation unit was set up for the supply of liquid inoculants: NPK providing consortium; BioGrow; P solubilizing bacteria; K solubilizing bacteria; Zn solubilizing bacteria and Rhizobium for different pulse crops.

 

Ø Biopriming of rice seeds with cyanobacteria: A biopriming technique was developed for the coating of rice seeds with potential cyanobacterial strains (Plectonema boreanum, Anabaena doliolum, Nostoc commune andan equi-proportional mix-culture of these three strains). Coating of rice seeds with cyanobacteria led to an increase of 5.3 to 7.9 % in grain yield among different varieties.

 

Ø Technology for long term storage of Cyanobacterial Cultures: Two different technologies were developed for long term storage of cyanobacteial cultures:

 

i. Culture storage on sterilized and dried Whatman filter paper strips: cultures were viable after 12 months.

ii. Culture storage on sterilized silica gel (mesh size 60-120): cultures retained its viability even after 18 months

Ø Mobile Apps for digital outreach: Mobile apps developed by ICAR-NBAIM and launched by Hon’ble Minister of Agriculture & Farmer’s Welfare, Sh. Radha Mohan Singh Ji on 15.03.17 in Krishi Unnati Mela

 

· MGRportal App: An android based mobile app of MGRportal for general search of microbial accessions by accession number or by name in NAIMCC database.

 

· Micromitra App: Another bilingual mobile app for farmers. It enlists various microbe based technologies developed at ICAR-NBAIM, Mau, U.P. and gives details about crops benefited, advantages, application, dosage, precautions and cost of each technology

 

Ø Microbial Genetic Resource Portal: It contains information on

· NAIMCC - National Agriculturally Important Microbial Culture Collection

· MGRR - Microbial Genomic Resource Repository

· Designated Culture Collections of India by NBA

· Information on AMAAS (Application of Microbes in Agriculture and Allied Sectors)

· Services offered by NAIMCC/NBAIM

· Guidelines for Registration of Elite Microbial Germplasm

· Accessibility of culture collection catalogues

· Passport Data Form for Microbial Deposition

 

Patents filed (Two)

a. Hillol Chakdar, Prem Lal Kashyap, Alok K Srivastava, Pragya Saxena (April 27, 2015) A method of high throughput fungal DNA template preparation and uses thereof. [Indian Patent Office No. 1170/DEL/2015]- Applicant: ICAR

b. Prem Lal Kashyap, Hillol Chakdar, Alok K Srivastava, Shalini Rai (May 07, 2015) A method of detection of Alternaria spp. and uses thereof. [Indian patent office No. 1287/DEL/2015.] Applicant: ICAR.

 

B. Application of Microorganisms in Agriculture and Allied Sectors (AMAAS)

 

Under AMAAS certain products were developed using the Agriculturally Important Microorganisms (AIMs):

1. Biocapsule: The novel invention of PGPR formulation as biocapsule is an easy and reliable technology of storing and delivering PGPR bioagents in hard gelatin capsule termed as biocapsule.

 

2. Talc based formulation: Developed and commercialized two Talc based- microbial formulations, namely, “IISR Biopower” (Bacillus amyloliquefaciens GRB-35) for ginger and “IISR Biomix” (consortium of Micrococcus luteusBRB3 and Enterobacter aerogenesBRB 13) for black pepper.

 

3. Bioformulation: Bacillus megaterium based bioformulation which could increase total dry matter weight of coconut seedlings up to 22-38% was branded 'KeraProbio' while Pseudomonas putida that could increase the total dry matter weight of cocoa seedlings up to 27-56% was branded 'Cocoa Probio'.

 

4. Mycorrhizae bioinoculant: The bioinoculant(KerAM) contains Claroideoglomus etunicatum, a AM fungi, having high potential to increase the plant growth parameters of coconut seedlings was released in 2014

 

5. Zinsol: Zinsol is a Pseudomonas based zinc solubilizer that can solubilize insoluble forms of zinc into available forms for uptake by crops. The formulation is effective in maize and other crops for enhancing yield and nutritive quality.

 

6. Products from Kinnow residues: Processes for various products from Kinnow residues, such as Kinnow face pack, toner, Kinnow peel candies, Kinnow peel wine, enzymatic-aided extraction of bio colours, etc. with microbiological interventions have been standardized. Further, processes for production of face care system and nutraceutical beverages from Kinnow peels are ready for licensing.

 

7. Commercial enzymes from mango pomace

8. Microbial pigments for food and textile industry

9. Consortium for Poly Aromatic Hydrocarbon (PAH) degradation

10. Bioethanol production from Kinnow waste (CIPHET).

 

11. Bioinoculants for soybean: 18% increase in grain yield in soybean was recorded in 100 field trials in Vidisha and Raisen districts of Madhya Pradesh due to inoculation of Bradyrhizobium japonicum R-33 and Bacillus megaterium P-3. More than 6 lakhs biofertilizer packets of these strains were supplied to farmers for various crops since 2009.

 

12. Bacterial and cyanobacterial consortia for rice –Wheat cropping system: Field based evaluation of a set of three bacterial and cyanobacterial strains recorded highest grain yield and harvest index along with basal application of ½ N+PK in wheat crop.

 

13. Bacillus subtilis for aerobic composting: An eco-friendly aerobic composting using Bacillus subtilis was developed and is being implemented in many residential apartments in Thrissur Corporation, Alapuzha municipality waste management system and other local bodies in Kerala.

 

14. Consortium to alleviate drought and heat stress: Two mixed inocula (mixture of three bacteria) for alleviating abiotic stress (Drought and Heat) in rainfed crops and field tested in maize, sorghum, and sunflower under rainfed conditions. The names of two inocula are as follows.

 

· CRIDA MI-I (Pseudomonas putida P7 + Paenibacillus favisporus-B30 + Pantoea agglomerans-G12)

· CRIDA MI-II (Pseudomonas putida P45 + Bacillus amyloliquefaciens B17 + Pantoea agglomerans G12)

15. DNA based diagnostic kits for the identification of Bacillus, Macrophomina phaseolina, Fusarium udum and R. solani.

16. Diagnostic markers for detection of Fusarium oxysporum and Alternaria spp.

 

17. Bt based technologies for biocontrol: Large area testing of DOR Bt-127 SC formulation on castor (2000 sq.m.) resulted in 98-99% decrease in population of Achaea janata and Spodoptera litura by 10 days after spray. Foliar damage before spray ranged 5-10% and increased to 20-25% by 10 DAS in control plot. In field trials on sunflower at ORS-Latur, DOR Bt-127 SC formulation was most effective in controlling Helicoverpa armigera and Thysanoplusia orichalcea followed by SC formulations of Bt-127 in combination with Nomuraea rileyi and Beauveria bassiana (79-82% decrease over control). All the formulation were superior to the chemical insecticide Profenophos (57.5% decrease over control).

 

18. Liquid Bt formulation:Liquid Bt formulations using indigenous strains of Bt (PDBC-BT1 & NBAII-BTG4) was developed. It is effective against pigeon pea pod borer (H. armigera- 30-40% increase in yield), Cauliflower DBM (Plutella xylostella) (83% reduction of pest and 19% increase in yield). Multi location trials have been completed under AICRP Biocontrol and data generated for CIB&RC registration.

 

19. Trichoderma based biopesticide: Trichoderma harzianum Th4d SC (Suspension Concentrate); Multi location field trials conducted and found effective againstPhytophthoraseedling blight, Macrophomina root rot, Fusarium wilt of safflower and castor; Gray mold of castor; Alternaria ster leaf blight and powdery mildew of sunflower. The technology is licensed to 3 firms; Patent filed and published

 

20. Trichodermaasperellum Ta DOR 7316 5% W: A thermotolerant, defense inducing and plant growth promoting strain in crops under drought conditions. Multi location field trials are being conducted at AICRP centres of Safflower and Castor during 2013-14. Patent application has been filed.

 

21. Fungicide & Salinity tolerant strains of Trichoderma harzianum: Salinity and carbendazim tolerant strains of HAR-4B and GJ-16B identified for groundnut, tomato and sorghum against soil borne pathogens. It can reduce disease incidence by 50-60% and increase yield by 10-20%.

 

22. Formulations of Beavuria bassiana, Metarhizium anisopliae, Lecanicillium spp and Paecilomyces fumosoroseus for management of certain sucking pests in vegetable crops.

 

23. Entopathogenic fungi (EPF) based formulations: Broad spectrum promising strains of EPF (Bb-5a, Ma-4and Vl-8) against Cowpea aphid (Aphis craccivora), Cabbage & Cauliflower aphid (Brevicoryne brassicae), Chilli & Brinjalaphid (Aphis gossypii) with 60-80% pest reduction and 15-20% increase in yield

 

24. Pseudomonas fluorescens based biopesticide formulation: DAPG producing, temperature, salinity and drought tolerant P. fluorescens developed for control of soil borne diseases of Groundnut and foliar diseases of Paddy. Multilocational trials conducted in Karnataka, Gujarat and Tamil Nadu (100 acres).

 

25. Consortium for rapid decomposition agro waste at low temperatures: Two potent lignocellulolytic bacteria: Bacillus megaterium and Paenibacillus polymyxa and two lignocellulolytic fungi: Penicillium sp.(SF3), and Mucor plumbeus (LF09) that produce cold active enzymes were used to develop a consortiumfor degradation of lignocellulolytic biomass at low temperature. The consortium successfully decomposed agricultural waste such as rice straw in 60 d during winter season in Indo-Gangetic plains (temperature ranging from 10 – 25 °C) and in 90 d at higher altitudes of Leh, Laddhak (temperature ranging from -10 – 5 °C). DRDO laboratory at Leh (DIHAR, Leh) has taken consortium for composting of more than 2 tons of leaf waste during 2016-17

 

Patents filed (Two)

c. Hillol Chakdar, Prem Lal Kashyap, Alok K Srivastava, Pragya Saxena (April 27, 2015) A method of high throughput fungal DNA template preparation and uses thereof. [Indian Patent Office No. 1170/DEL/2015]- Applicant: ICAR

 

d. Prem Lal Kashyap, Hillol Chakdar, Alok K Srivastava, Shalini Rai (May 07, 2015) A method of detection of Alternaria spp. and uses thereof. [Indian patent office No. 1287/DEL/2015.] Applicant: ICAR.

 

C. Application of Microorganisms in Agriculture and Allied Sectors (AMAAS)

 

Under AMAAS certain products were developed using the Agriculturally Important Microorganisms (AIMs):

26. Biocapsule: The novel invention of PGPR formulation as biocapsule is an easy and reliable technology of storing and delivering PGPR bioagents in hard gelatin capsule termed as biocapsule.

 

27. Talc based formulation: Developed and commercialized two Talc based- microbial formulations, namely, “IISR Biopower” (Bacillus amyloliquefaciens GRB-35) for ginger and “IISR Biomix” (consortium of Micrococcus luteusBRB3 and Enterobacter aerogenesBRB 13) for black pepper.

 

28. Bioformulation: Bacillus megaterium based bioformulation which could increase total dry matter weight of coconut seedlings up to 22-38% was branded 'KeraProbio' while Pseudomonas putida that could increase the total dry matter weight of cocoa seedlings up to 27-56% was branded 'Cocoa Probio'.

 

29. Mycorrhizae bioinoculant: The bioinoculant(KerAM) contains Claroideoglomus etunicatum, a AM fungi, having high potential to increase the plant growth parameters of coconut seedlings was released in 2014

 

30. Zinsol: Zinsol is a Pseudomonas based zinc solubilizer that can solubilize insoluble forms of zinc into available forms for uptake by crops. The formulation is effective in maize and other crops for enhancing yield and nutritive quality.

 

31. Products from Kinnow residues: Processes for various products from Kinnow residues, such as Kinnow face pack, toner, Kinnow peel candies, Kinnow peel wine, enzymatic-aided extraction of bio colours, etc. with microbiological interventions have been standardized. Further, processes for production of face care system and nutraceutical beverages from Kinnow peels are ready for licensing.

 

32. Commercial enzymes from mango pomace

33. Microbial pigments for food and textile industry

34. Consortium for Poly Aromatic Hydrocarbon (PAH) degradation

35. Bioethanol production from Kinnow waste (CIPHET).

 

36. Bioinoculants for soybean: 18% increase in grain yield in soybean was recorded in 100 field trials in Vidisha and Raisen districts of Madhya Pradesh due to inoculation of Bradyrhizobium japonicum R-33 and Bacillus megaterium P-3. More than 6 lakhs biofertilizer packets of these strains were supplied to farmers for various crops since 2009.

 

37. Bacterial and cyanobacterial consortia for rice –Wheat cropping system: Field based evaluation of a set of three bacterial and cyanobacterial strains recorded highest grain yield and harvest index along with basal application of ½ N+PK in wheat crop.

 

38. Bacillus subtilis for aerobic composting: An eco-friendly aerobic composting using Bacillus subtilis was developed and is being implemented in many residential apartments in Thrissur Corporation, Alapuzha municipality waste management system and other local bodies in Kerala.

 

39. Consortium to alleviate drought and heat stress: Two mixed inocula (mixture of three bacteria) for alleviating abiotic stress (Drought and Heat) in rainfed crops and field tested in maize, sorghum, and sunflower under rainfed conditions. The names of two inocula are as follows.

 

· CRIDA MI-I (Pseudomonas putida P7 + Paenibacillus favisporus-B30 + Pantoea agglomerans-G12)

· CRIDA MI-II (Pseudomonas putida P45 + Bacillus amyloliquefaciens B17 + Pantoea agglomerans G12)

40. DNA based diagnostic kits for the identification of Bacillus, Macrophomina phaseolina, Fusarium udum and R. solani.

41. Diagnostic markers for detection of Fusarium oxysporum and Alternaria spp.

 

42. Bt based technologies for biocontrol: Large area testing of DOR Bt-127 SC formulation on castor (2000 sq.m.) resulted in 98-99% decrease in population of Achaea janata and Spodoptera litura by 10 days after spray. Foliar damage before spray ranged 5-10% and increased to 20-25% by 10 DAS in control plot. In field trials on sunflower at ORS-Latur, DOR Bt-127 SC formulation was most effective in controlling Helicoverpa armigera and Thysanoplusia orichalcea followed by SC formulations of Bt-127 in combination with Nomuraea rileyi and Beauveria bassiana (79-82% decrease over control). All the formulation were superior to the chemical insecticide Profenophos (57.5% decrease over control).

 

43. Liquid Bt formulation:Liquid Bt formulations using indigenous strains of Bt (PDBC-BT1 & NBAII-BTG4) was developed. It is effective against pigeon pea pod borer (H. armigera- 30-40% increase in yield), Cauliflower DBM (Plutella xylostella) (83% reduction of pest and 19% increase in yield). Multi location trials have been completed under AICRP Biocontrol and data generated for CIB&RC registration.

 

44. Trichoderma based biopesticide: Trichoderma harzianum Th4d SC (Suspension Concentrate); Multi location field trials conducted and found effective againstPhytophthoraseedling blight, Macrophomina root rot, Fusarium wilt of safflower and castor; Gray mold of castor; Alternaria ster leaf blight and powdery mildew of sunflower. The technology is licensed to 3 firms; Patent filed and published

 

45. Trichodermaasperellum Ta DOR 7316 5% W: A thermotolerant, defense inducing and plant growth promoting strain in crops under drought conditions. Multi location field trials are being conducted at AICRP centres of Safflower and Castor during 2013-14. Patent application has been filed.

 

46. Fungicide & Salinity tolerant strains of Trichoderma harzianum: Salinity and carbendazim tolerant strains of HAR-4B and GJ-16B identified for groundnut, tomato and sorghum against soil borne pathogens. It can reduce disease incidence by 50-60% and increase yield by 10-20%.

 

47. Formulations of Beavuria bassiana, Metarhizium anisopliae, Lecanicillium spp and Paecilomyces fumosoroseus for management of certain sucking pests in vegetable crops.

 

48. Entopathogenic fungi (EPF) based formulations: Broad spectrum promising strains of EPF (Bb-5a, Ma-4and Vl-8) against Cowpea aphid (Aphis craccivora), Cabbage & Cauliflower aphid (Brevicoryne brassicae), Chilli & Brinjalaphid (Aphis gossypii) with 60-80% pest reduction and 15-20% increase in yield

 

49. Pseudomonas fluorescens based biopesticide formulation: DAPG producing, temperature, salinity and drought tolerant P. fluorescens developed for control of soil borne diseases of Groundnut and foliar diseases of Paddy. Multilocational trials conducted in Karnataka, Gujarat and Tamil Nadu (100 acres).

 

50. Consortium for rapid decomposition agro waste at low temperatures: Two potent lignocellulolytic bacteria: Bacillus megaterium and Paenibacillus polymyxa and two lignocellulolytic fungi: Penicillium sp.(SF3), and Mucor plumbeus (LF09) that produce cold active enzymes were used to develop a consortiumfor degradation of lignocellulolytic biomass at low temperature. The consortium successfully decomposed agricultural waste such as rice straw in 60 d during winter season in Indo-Gangetic plains (temperature ranging from 10 – 25 °C) and in 90 d at higher altitudes of Leh, Laddhak (temperature ranging from -10 – 5 °C). DRDO laboratory at Leh (DIHAR, Leh) has taken consortium for composting of more than 2 tons of leaf waste during 2016-17